El Rol de la saliva en la identificación SARS-CoV-2
DOI:
https://doi.org/10.31984/oactiva.v7i2.572Keywords:
Sars-Cov-2; Covid-19; Saliva tests; Sars-Cov-2 diagnosis; betacoronavirus.Abstract
SUMMARY:
Objective: The objective of this review was to evaluate the role of saliva tests for the diagnosis of Covid-19. Methodology: This is a literature review study performed between July and November of 2020. The languages used were Spanish and English. We used multiple search engines such as PubMed, Scopus, Redalyc, Google Scholar, Ebscohost, SciELO, and Springer to obtain scientific articles on the subject. The article also includes short systematic researches, pilot clinical trials, literature reviews, letters to the editor, and information from an electronic document. Results: The results indicate that the diagnosis of Sars-Cov-2 in the saliva is a non-invasive procedure that allows a rapid and early detection of the disease. This method also enables monitoring while reducing the risk of nosocomial transmission. Thus, it is useful in the replacement of nasopharyngeal swabs when they are contraindicated. In addition, salivary specimens are reliable in the molecular analysis due to their self-collection which also minimizes errors during sample transfer. The cost is lower and is not invasive. This is particularly important if epidemiological procedures are performed with limited resources. Conclusions: The paper's main conclusions are based on new evidence that shows saliva as the fluid that offers several benefits for the diagnosis of Covid-19 including the simplicity of the process, the cost, and especially the ability to detect the disease in acute phases.
KEYWORDS: Sars-Cov-2; Covid-19; Saliva tests; Sars-Cov-2 diagnosis; betacoronavirus.
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References
Suárez S; Campuzano R; Dona M; Garrido E, Gimenez M. Recomendaciones para prevención y control de infecciones por SARS-CoV-2 en odontología. 2020; 22 (2): 5-32. DOI https://doi.org/10.29166/odontologia.vol22.n2.2020-5-32
Temas de salud [Internet]. Organización Mundial de la Salud. 2020 [citado 18 noviembre 2020]. Disponible en: https://www.who.int/es/emergencies/diseases/novel-coronavirus-2019/technical-guidance/naming-the-coronavirus-disease-(covid-2019)-and-the-virus-that-causes-it
Melián A; Calcumil P; Boin C; Carrasco R. Detección de COVID -19 (SARS-CoV-2) Mediante la Saliva: Una Alternativa Diagnóstica poco Invasiva. Int. J. Odontostomat. 2020; 14(3): 316-320. DOI 10.1177/0022034520960070
Fernandes L.L. et al. Saliva in the Diagnosis of COVID-19: A Review and New Research Directions, J Den Res. 2020; 99(13):1435–1443. DOI 10.1177/0022034520960070.
Pasomsub E., S.P. Watcharananan, K. Boonyawat, P. Janchompoo. et al. Saliva sample as a non-invasive specimen for the diagnosis of coronavirus disease 2019: a cross-sectional study. Clinical Microbiology and Infection. 2020. DOI https://doi.org/10.2016/j.cmi.2020.05.001
Sapkota D, Søland TM, Galtung HK, et al COVID-19 salivary signature: diagnostic and research opportunities. J Clin Pathol 2020;0:1–6 doi: 10.1136/jclinpath-2020-206834
Pérez-Domínguez, M., Pérez-Y; Ibarra L. SARS-CoV-2 en saliva: potencial vía de contagio e implicaciones en el tratamiento del paciente odontológico. Odous Científica. 2020; 21(1): 77-88 77. Disponible en https://www.researchgate.net/publication/340540327_SARS-CoV-2_en_saliva_potencial_via_de_contagio_e_implicaciones_en_el_tratamiento_del_paciente_odontologico
Dadlani S. Sars-CoV-2 Transmission in a Dental Practice in Spain: After the Outbreak. Int J of Dent. 2020; 2020:1-4 DOI https://doi.org/10.1155/2020/8828616
Pascolo L; Zupin L; Melato M; Tricarico PM; Crovella S. TMPRSS2 and ACE2 Coexpression in SARS-CoV-2 Salivary Glands Infection. J Dent Res. 2020; 99(10). 1120-1121. DOI 10.1177/0022034520933589
Jacobs M; Van Eeckhoutte HP; Wijnant SRA; et al. Increased expression of ACE2, the SARS-CoV-2 entry receptor, in alveolar and bronchial epithelium of smokers and COPD subjects. Eur Respir J 2020; 1-10 https://doi.org/10.1183/13993003.02378-2020).
Xu H; Zhong L; Deng J; Peng J; Dan H; Zeng X; Li T; Chen Q. High Expression of ACE2 receptor of 2019-nCoV on the ephitelial cells of oral mucosa. IJOS.2020;12:8;1-5. https://doi.org/10.1038/s41368-020-0074-x
Ghafouri S; Noroozi R; Davood M; Branicki W; Pospiech E; Sayad A; Pyrc K; et al. : Angiotensin converting enzyme: A review on expression profile and its association with human disorders with special focus on SARS-CoV-2 infection. Vasc Ph. 2020; 16680; 1-10. DOI https://doi.org/10.1016/j.vph.2020.106680
Yuki K; Fujiogi M; Koutsogiannaki S. Covid-19 pathophysioly: A review. Clin Immunol.2020; 215: 108427. https://doi.org/10.1016/j.clim.2020.108427
Morales E. La Saliva como Biomuestra para Diagnóstico de Infección por SARS-CoV-2: Una Revisión. Int. J. Odontostomat.,2020; 14(3):327-330. DOI http://dx.doi.org/10.4067/S0718-381X2020000300327
Perez M; Perez YL. SARS-CoV-2 en saliva: potencial vía de contagio e implicaciones en el tratamiento del paciente odontologico. Odus Cientifica. 2020;21:1 77-88. Disponible en https://www.researchgate.net/publication/340540327_SARS-CoV-2_en_saliva_potencial_via_de_contagio_e_implicaciones_en_el_tratamiento_del_paciente_odontologico.
Wiersinga WJ;, Rhodes A,; Cheng AC;, Peacock SJ;, Prescott HC. Pathophysiology, Transmission, Diagnosis, and Treatment of Coronavirus Disease 2019 (COVID-19): A Review. JAMA. 2020;324(8):782–793. https://edhub.ama-assn.org/jn-learning/module/2768391
Fernandes LL, Pacheco VB, Borges L, et al. Saliva in the Diagnosis of COVID-19: A Review and New Research Directions. Journal of Dental Research. 2020;99(13):1435-1443
Pacheco M., Pacheco J., Hernández A., Cázares de León F. "Consideraciones sobre el diagnóstico de COVID-19 y el papel del diagnóstico salival.". Revista ADM. 2020; 77(4): 191-196. DOI: 10.35366/95112
Czumbel LM;, Kiss S;, Farkas N;, Mandel I;, Hegyi A;, Nagy Á; et al. Saliva as a Candidate for COVID-19 Diagnostic Testing: A Meta-Analysis. Front Med. 2020; 4(7):465. DOI 10.3389/fmed.2020.00465
Sabino R., Gomes A., Siqueira L. Coronavirus COVID-19 impacts to dentistry and potential salivary diagnosis. Clin Oral Invest. 2020; 24: 1619–1621. DOI: https://www.doi.org/10.1007/ s00784-020-03248-x
Li Y; Ren B; Peng X., et al. Saliva is a non‐negligible factor in the spread of COVID‐19. Mol Oral Microbiol. 2020;35: 141– 145. Disponible en https://onlinelibrary.wiley.com/doi/epdf/10.1111/omi.12289
Procop GW; Shrestha NK,; Vogel S;, Van Sickle K;, Harrington S;, Rhoads DD;, et al. A direct comparison of enhanced saliva to nasopharyngeal swab for the detection of SARS-CoV-2 in symptomatic patients. J Clin Microbiol. 2020; 58(11)1946-20 DOI 10.1128/JCM.01946-20
To, K. K.; Tsang, O. T.; Chik-Yan Yip, C.; Chan, K. H.; Wu, T. C.; Chan, J. M. C.; Leung, W. S.; Chik, T. S.; Choi, C. Y.; Kandamby, D. H.; et al. Consistent detection of 2019 novel coronavirus in saliva. Clin Infect Dis. 2020;71(15):841–3 https://doi.org/10.1093/cid/ciaa149
Baghizadeh Fini M. Oral saliva and COVID-19. Oral Oncol. 2020; 108: 104821. DOI 10.1016/j.oraloncology.2020.104821
Azzi, L., et. al. Diagnostic Salivary Tests for SARS-CoV-2. Journal of Dental Research.2020:1 https://doi.org/10.1177/0022034520969670
Hung K., Sun Y., Chen B., et al. New COVID-19 saliva-based test: How good is it compared with the current nasopharyngeal or throat swab test?. J Chin Med Assoc. 2020; 83(10): 891-894. DOI 10.1097/JCMA.0000000000000396
Nunes S., Souza D., Martins E. et al. Saliva is a reliable, non-invasive specimen for SARS-CoV-2 detection. Braz J Infect Dis. 2020; 24(5): 422-427. https://doi.org/10.1016/j.bjid.2020.08.001
Takeuchi Y., Furuchi M., Kamimoto A., Honda K., Matsumura H., Kobayashi R. Saliva-based PCR tests for SARS-CoV-2 detection. Journal of Oral Science.2020;62(3):350-351 https://doi.org/10.2334/josnusd.20-0267
Kandel C, Zheng J, McCready J, Serbanescu MA, Racher H, Desaulnier M, Powis JE, Vojdani K, Finlay L, Sheldrake E, Vermeiren C, Katz K, McGeer A, Kozak R, Goneau LW. Detection of SARS-CoV-2 from Saliva as Compared to Nasopharyngeal Swabs in Outpatients. Viruses. 2020 17;12(11):1314. doi: 10.3390/v12111314. PMID: 33212817; PMCID: PMC7697440.
[Internet]. Asociación Española de Vacunología. 2020 [citado 18 noviembre 2020]. Disponible en: https://www.vacunas.org/autorizada-una-prueba-de-saliva-para-el-diagnostico-de-la-covid-19/
Khurshi Z.; Asiri, F; Y. I; Al W.; H. Human Saliva: Non-Invasive Fluid for Detecting Novel Coronavirus (2019-nCoV). Int. J. Environ. Res. Public Health, 17(7):2225, 2020. DOI: https://www.doi.org/10.3390/ijerph17072225
Melián R. A.; CALCUMIL, H. P.; BOIN, B. C. & CARRASCO, S. R. Detección de COVID-19 (SARS-CoV-2) mediante la saliva: Una alternativa diagnóstica poco invasiva. Int. J. Odontostomat., 14(3):316-320, 2020. DOI http://dx.doi.org/10.4067/S0718-381X2020000300316
To, K. K.; Lu, L.; Yip, C. C.; Poon, R. W.; Fung, A. M.; Cheng, A.; Lui, D. H.; Ho, D. T.; Hung, I. F.; Chan, K. H.; et al. Additional molecular testing of saliva specimens improves the detection of respiratory viruses. Emerg. Microbes Infect.. 6(6):e 49, 2017 DOI 10.1038/emi.2017.35
Wang, W. K.; Chen, S. Y.; Liu, I. J.; Chen, Y. C.; Chen, H. L.; Yang, C.F.; Chen, P. J.; Yeh, S. H.; Kao, C. L.; Huang, L. M.; et al. Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis. Emerg. Infect. Dis., 10(7):1213-9, 2004. 10.3201/eid1007.031113
Fang, Z.; Zhang, Y.; Hang, C.; Li, S.; Ai, J. & Zhang, W. Comparisons of viral shedding time of SARS-CoV-2 of different samples in ICU and non-ICU patients. J. Infect., 2020b. DOI: https://www.doi.org/ 10.1016/j.jinf.2020.03.013
To, K. K.; Tsang, O. T.; Leung, W. S.; Tam, A. R.; Wu, T. C.; Lung, D.C.; Yip, C. C.; Cai, J. P.Chan, J. M.; Chik, T. S.; et al. Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study. Lancet Infect. Dis., 2020b. DOI: https://www.doi.org/10.1016/S1473-3099(20)30196-1
Fan J, Yu F, Wang X , et al. Hock-a-loogie saliva as a diagnostic specimen for SARS-CoV-2 by a PCR-based assay: A diagnostic validity study, Clinica Chimica Acta. 2020; 511: 177-180. DOI https://doi.org/10.1016/j.cca.2020.10.004.
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