MOLECULAR BIOLOGY TECHNIQUES FOR RESEARCH IN DENTISTRY AND ORAL BIOLOGY (PART TWO)
DOI:
https://doi.org/10.31984/oactiva.v4i3.395Keywords:
Molecular biology, RNA, DNA, Oral biologyAbstract
Molecular Biology is dedicated to the study of the structure and function of two important biomolecules - proteins and nucleic acids - and the interactions established between them. In the first article of this series, we presented some of the “molecular” techniques and their applications in the dental field. In this second article, we present the nucleic acid sequencing methods that have allowed us to know in depth the details about the way in which genetic information is stored and decoded in living beings. The sequences obtained from the study of genes and genomes are useful, not only to identify and classify living beings (among which disease-causing microorganisms) but also to understand the genetic basis of many diseases. We also present the massive parallel sequencing strategies (also called Next Generation Sequencing Technologies, or NGS) that have allowed us to elucidate the complex structure and composition of the communities of microorganisms that colonize the oral cavity.
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